Hotspot

ccsA-ycf1

Genome · 31447-31481 · 35 bp

Back to Callitropsis

Pi 0.0571
MarkerSeek score 69.80
Primer available yes
Label ccsA-ycf1

Feature Metadata

label_name
ccsA-ycf1
feature_type
igs
region
Genome
length_bp
35
variable_sites
2
pi
0.0571
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
69.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA-ycf1_p1 GACAAAAACCGGCGATTGTT TTGGAAGTCTTCAAGAAGGT 45.0 40.0 57.9 54.1 167 bp 1.0 48.7
ccsA-ycf1_p2 ACAAAAACCGGCGATTGTTG TTGGAAGTCTTCAAGAAGGT 45.0 40.0 58.2 54.1 166 bp 1.0 48.5
ccsA-ycf1_p3 CGGCGATTGTTGCTTCTCTA TTGGAAGTCTTCAAGAAGGT 50.0 40.0 58.4 54.1 158 bp 1.0 46.9
ccsA-ycf1_p4 GACAAAAACCGGCGATTGTT ATTGGAAGTCTTCAAGAAGGT 45.0 38.1 57.9 54.7 168 bp 1.0 45.8
ccsA-ycf1_p5 ACAAAAACCGGCGATTGTTG ATTGGAAGTCTTCAAGAAGGT 45.0 38.1 58.2 54.7 167 bp 1.0 45.6