Hotspot

clpP-trnP-GGG

Genome · 29744-30020 · 277 bp

Back to Callitropsis

Pi 0.0289

MarkerSeek score 55.50

Primer available yes

Label clpP-trnP-GGG

Feature Metadata

label_name: clpP-trnP-GGG
feature_type: igs
region: Genome
length_bp: 277
variable_sites: 8
pi: 0.0289
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP-trnP-GGG_p1	GAGCTTTCCTTCTACACGGG	CGAATAGAGCGAACGGGAAA	55.0	50.0	58.0	58.1	1270-1282 bp	1.0	81.1
clpP-trnP-GGG_p2	GGAGCTTTCCTTCTACACGG	CGAATAGAGCGAACGGGAAA	55.0	50.0	58.0	58.1	1271-1283 bp	1.0	81.1
clpP-trnP-GGG_p3	GAGCTTTCCTTCTACACGGG	GCGATGCGAGACTGCTAATA	55.0	50.0	58.0	57.9	763 bp	0.5	59.5
clpP-trnP-GGG_p4	GGAGCTTTCCTTCTACACGG	GCGATGCGAGACTGCTAATA	55.0	50.0	58.0	57.9	764 bp	0.5	59.5
clpP-trnP-GGG_p5	GGGAGCTTTCCTTCTACACG	GCGATGCGAGACTGCTAATA	55.0	50.0	58.0	57.9	765 bp	0.5	59.5