Hotspot

rps12-clpP

Genome · 27904-29152 · 1249 bp

Back to Callitropsis

Pi 0.0323

MarkerSeek score 81.70

Primer available yes

Label rps12-clpP

Feature Metadata

label_name: rps12-clpP
feature_type: igs
region: Genome
length_bp: 1249
variable_sites: 38
pi: 0.0323
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 81.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-clpP_p1	GCATACTCCCTTCCGTTGAG	GCAGGTTGTTTTTCTAGCGC	55.0	50.0	58.1	58.3	1428-1479 bp	1.0	83.1
rps12-clpP_p2	TTTCTTATCGGCTGTCTCGC	GCAGGTTGTTTTTCTAGCGC	50.0	50.0	58.1	58.3	1376-1427 bp	1.0	83.0
rps12-clpP_p3	CTTATCGGCTGTCTCGCATT	GCAGGTTGTTTTTCTAGCGC	50.0	50.0	58.1	58.3	1373-1424 bp	1.0	82.5
rps12-clpP_p4	TATCGGCTGTCTCGCATTTC	GCAGGTTGTTTTTCTAGCGC	50.0	50.0	58.1	58.3	1371-1422 bp	1.0	82.5
rps12-clpP_p5	TTATCGGCTGTCTCGCATTT	GCAGGTTGTTTTTCTAGCGC	45.0	50.0	57.7	58.3	1372-1423 bp	1.0	81.2