Hotspot

psaJ-rpl33

Genome · 25484-25798 · 315 bp

Back to Callitropsis

Pi 0.0317

MarkerSeek score 71.10

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: Genome
length_bp: 315
variable_sites: 10
pi: 0.0317
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	ATATCTTTCCACAGCGCCTG	TTACATTTCCACCCTTGGCC	50.0	50.0	58.0	58.1	449 bp	1.0	80.9
psaJ-rpl33_p2	CATATCTTTCCACAGCGCCT	TTACATTTCCACCCTTGGCC	50.0	50.0	58.0	58.1	450 bp	1.0	80.9
psaJ-rpl33_p3	ACATATCTTTCCACAGCGCC	TTACATTTCCACCCTTGGCC	50.0	50.0	58.3	58.1	451 bp	1.0	78.6
psaJ-rpl33_p4	GCGCCTGTGTTAGCTACTTT	TTACATTTCCACCCTTGGCC	50.0	50.0	58.6	58.1	436 bp	1.0	76.8
psaJ-rpl33_p5	ATATCTTTCCACAGCGCCTG	CATTTCCACCCTTGGCCAT	50.0	52.6	58.0	58.0	446 bp	1.0	73.4