Hotspot

ycf4-cemA

Genome · 20222-20688 · 467 bp

Back to Callitropsis

Pi 0.015

MarkerSeek score 64.60

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: Genome
length_bp: 467
variable_sites: 7
pi: 0.015
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	ATTTGACCTTGCGCGAAATG	TGGCAACTTCCAATTCGTGA	45.0	45.0	58.0	58.0	647 bp	1.0	80.5
ycf4-cemA_p2	GAAAATTTGACCTTGCGCGA	TGGCAACTTCCAATTCGTGA	45.0	45.0	58.0	58.0	651 bp	1.0	80.3
ycf4-cemA_p3	AGAAAATTTGACCTTGCGCG	TGGCAACTTCCAATTCGTGA	45.0	45.0	57.9	58.0	652 bp	1.0	80.2
ycf4-cemA_p4	ATTTGACCTTGCGCGAAATG	CTCGTTAGCTCTGCCCAAAA	45.0	50.0	58.0	58.2	606 bp	1.0	79.2
ycf4-cemA_p5	GAAAATTTGACCTTGCGCGA	CTCGTTAGCTCTGCCCAAAA	45.0	50.0	58.0	58.2	610 bp	1.0	79.0