Hotspot

infA-rpl36

Genome · 11649-11683 · 35 bp

Back to Callitropsis

Pi 0.0286

MarkerSeek score 63.80

Primer available yes

Label infA-rpl36

Feature Metadata

label_name: infA-rpl36
feature_type: igs
region: Genome
length_bp: 35
variable_sites: 1
pi: 0.0286
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
infA-rpl36_p1	CATCCCGTCAAACGAAAGGA	CTACGAATTAGGCGGCACTT	50.0	50.0	58.2	58.1	136 bp	1.0	78.5
infA-rpl36_p2	CCCGTCAAACGAAAGGATGA	CTACGAATTAGGCGGCACTT	50.0	50.0	58.2	58.1	133 bp	1.0	78.5
infA-rpl36_p3	TCCCGTCAAACGAAAGGATG	CTACGAATTAGGCGGCACTT	50.0	50.0	58.2	58.1	134 bp	1.0	78.5
infA-rpl36_p4	ACGAAAGGATGATGCTACCG	CTACGAATTAGGCGGCACTT	50.0	50.0	57.8	58.1	125 bp	1.0	78.3
infA-rpl36_p5	GATGATGCTACCGATGAGGC	CTACGAATTAGGCGGCACTT	55.0	50.0	58.3	58.1	118 bp	1.0	77.9