Hotspot

accD-rpl23

Genome · 4083-5324 · 1242 bp

Back to Callitropsis

Pi 0.0174

MarkerSeek score 71.40

Primer available yes

Label accD-rpl23

Feature Metadata

label_name: accD-rpl23
feature_type: igs
region: Genome
length_bp: 1242
variable_sites: 19
pi: 0.0174
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 71.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-rpl23_p1	CATGGTTCCACGAGCTCTTT	TTGATCCCGAATCGACATTT	50.0	40.0	58.2	55.1	1333-1409 bp	1.0	60.8
accD-rpl23_p2	CATGGTTCCACGAGCTCTTT	TTTGATCCCGAATCGACATT	50.0	40.0	58.2	55.1	1334-1410 bp	1.0	60.8
accD-rpl23_p3	CATGGTTCCACGAGCTCTTT	TTTGATCCCGAATCGACATTT	50.0	38.1	58.2	56.0	1334-1410 bp	1.0	59.3
accD-rpl23_p4	CATGGTTCCACGAGCTCTTT	ATTTGATCCCGAATCGACAT	50.0	40.0	58.2	54.9	1335-1411 bp	1.0	58.9
accD-rpl23_p5	ATGGTTCCACGAGCTCTTTT	TTGATCCCGAATCGACATTT	45.0	40.0	57.4	55.1	1332-1408 bp	1.0	57.7