Hotspot

rps14

Genome · 64437-64739 · 303 bp

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Pi 0.483

MarkerSeek score 73.50

Primer available yes

Label rps14

Feature Metadata

label_name: rps14
feature_type: gene
region: Genome
length_bp: 303
variable_sites: 145
pi: 0.483
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 73.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps14_p1	TTGAAACTCTTGCATGGGCT	AAGGTTATGAGCCTTGCGAG	45.0	50.0	58.0	58.0	775 bp	0.5	59.8
rps14_p2	TTGAAACTCTTGCATGGGCT	GAACCCGTGTCTTCAAGGTT	45.0	50.0	58.0	58.0	789 bp	0.5	59.7
rps14_p3	TTGAAACTCTTGCATGGGCT	CAAATGGGGTACATCGAGCA	45.0	50.0	58.0	58.0	690 bp	0.5	59.7
rps14_p4	TTGAAACTCTTGCATGGGCT	TTGAACCCGTGTCTTCAAGG	45.0	50.0	58.0	58.0	791 bp	0.5	59.7
rps14_p5	TTGAAACTCTTGCATGGGCT	ACATCGAGCAATCCTTTCCC	45.0	50.0	58.0	58.0	680 bp	0.5	59.6