Hotspot

psbC

Genome · 65932-67353 · 1422 bp

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Pi 0.479

MarkerSeek score 80.90

Primer available yes

Label psbC

Feature Metadata

label_name: psbC
feature_type: gene
region: Genome
length_bp: 1422
variable_sites: 582
pi: 0.479
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.85
markerseek_score: 80.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC_p1	GGCTCTGGTCTTGAAAACCA	GAGATTCGTGCAGCAGAAGA	50.0	50.0	58.0	58.0	1725 bp	0.5	59.8
psbC_p2	TGGCTCTGGTCTTGAAAACC	GAGATTCGTGCAGCAGAAGA	50.0	50.0	58.0	58.0	1726 bp	0.5	59.8
psbC_p3	GGCTCTGGTCTTGAAAACCA	GTGCTGCTCTTCTATGTGCT	50.0	50.0	58.0	58.0	2012 bp	0.5	59.7
psbC_p4	TGGCTCTGGTCTTGAAAACC	GTGCTGCTCTTCTATGTGCT	50.0	50.0	58.0	58.0	2013 bp	0.5	59.7
psbC_p5	GGCTCTGGTCTTGAAAACCA	GTTGGAGTAGTTGGCCTAGC	50.0	55.0	58.0	58.0	1779 bp	0.5	59.6