Hotspot

trnK-UUU-chlB

Genome · 112660-113599 · 940 bp

Back to Callitris

Pi 0.0832

MarkerSeek score 82.80

Primer available yes

Label trnK-UUU-chlB

Feature Metadata

label_name: trnK-UUU-chlB
feature_type: igs
region: Genome
length_bp: 940
variable_sites: 76
pi: 0.0832
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 82.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-chlB_p1	AGTCGCACTTAAAAGCCGAG	TCGAAGTAATGTACGCAGCT	50.0	45.0	58.6	57.1	1026-1178 bp	1.0	75.4
trnK-UUU-chlB_p2	AGTCGCACTTAAAAGCCGAG	GTCGAAGTAATGTACGCAGC	50.0	50.0	58.6	57.0	1027-1179 bp	1.0	75.1
trnK-UUU-chlB_p3	TAGTCGCACTTAAAAGCCGA	TCGAAGTAATGTACGCAGCT	45.0	45.0	57.3	57.1	1027-1179 bp	1.0	74.2
trnK-UUU-chlB_p4	TCGCACTTAAAAGCCGAGTA	TCGAAGTAATGTACGCAGCT	45.0	45.0	57.3	57.1	1024-1176 bp	1.0	74.2
trnK-UUU-chlB_p5	TCGCACTTAAAAGCCGAGTA	GTCGAAGTAATGTACGCAGC	45.0	50.0	57.3	57.0	1025-1177 bp	1.0	73.9