Hotspot

ycf3-psaA

Genome · 59318-59851 · 534 bp

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Pi 0.315

MarkerSeek score 81.20

Primer available yes

Label ycf3-psaA

Feature Metadata

label_name: ycf3-psaA
feature_type: igs
region: Genome
length_bp: 534
variable_sites: 146
pi: 0.315
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 81.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-psaA_p1	TGAAAGTTCGGGCTGATACG	CTTTGCGAGAGACCTCTTCC	50.0	55.0	58.0	58.0	1350 bp	0.5	59.9
ycf3-psaA_p2	TGAAAGTTCGGGCTGATACG	AACGCTGCAAAGATCAATGC	50.0	45.0	58.0	58.0	1648 bp	0.5	59.9
ycf3-psaA_p3	TGAAAGTTCGGGCTGATACG	CCAATTGCGGTGCAGTAAAG	50.0	50.0	58.0	58.0	1627 bp	0.5	59.8
ycf3-psaA_p4	TGAAAGTTCGGGCTGATACG	GTATTTGGTGTCCTGCCCAA	50.0	50.0	58.0	58.1	1788 bp	0.5	59.3
ycf3-psaA_p5	AGAACAGGCCATTCAACAGG	CTTTGCGAGAGACCTCTTCC	50.0	55.0	58.1	58.0	890 bp	0.5	59.3