primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
ycf2-ndhB_p1	ycf2-ndhB	1	GTCTTGTACAGGCGGCAATA	ATTCTGGTGTGGATGGCAAG	20	20	50.0	50.0	58.0	58.2	13.1	0.0	0.175174	29209	29953	1652-1652	0.500000	58.6
ycf2-ndhB_p2	ycf2-ndhB	2	GTCTTGTACAGGCGGCAATA	CTGCTTCAGCTTTAGCCACT	20	20	50.0	50.0	58.0	58.2	13.1	12.9	0.199900	29209	29953	2088-2088	0.500000	58.4
ycf2-ndhB_p3	ycf2-ndhB	3	GTCTTGTACAGGCGGCAATA	TCAGTCTACGTACGGGAACA	20	20	50.0	50.0	58.0	57.8	13.1	21.1	0.202020	29209	29953	1797-1797	0.500000	58.4
ycf2-ndhB_p4	ycf2-ndhB	4	GTCTTGTACAGGCGGCAATA	TGTTCAGTCTACGTACGGGA	20	20	50.0	50.0	58.0	57.8	13.1	21.1	0.202020	29209	29953	1800-1800	0.500000	58.4
ycf2-ndhB_p5	ycf2-ndhB	5	ACAGGCGGCAATATCTTTGT	ATTCTGGTGTGGATGGCAAG	20	20	45.0	50.0	57.9	58.2	13.1	0.0	0.285402	29209	29953	1645-1645	0.500000	57.7
ycf3-psaA_p1	ycf3-psaA	1	TGAAAGTTCGGGCTGATACG	CTTTGCGAGAGACCTCTTCC	20	20	50.0	55.0	58.0	58.0	0.0	0.6	0.007779	59318	59851	1350-1350	0.500000	59.9
ycf3-psaA_p2	ycf3-psaA	2	TGAAAGTTCGGGCTGATACG	AACGCTGCAAAGATCAATGC	20	20	50.0	45.0	58.0	58.0	0.0	14.0	0.017885	59318	59851	1648-1648	0.500000	59.9
ycf3-psaA_p3	ycf3-psaA	3	TGAAAGTTCGGGCTGATACG	CCAATTGCGGTGCAGTAAAG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.023329	59318	59851	1627-1627	0.500000	59.8
ycf3-psaA_p4	ycf3-psaA	4	TGAAAGTTCGGGCTGATACG	GTATTTGGTGTCCTGCCCAA	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.086711	59318	59851	1788-1788	0.500000	59.3
ycf3-psaA_p5	ycf3-psaA	5	AGAACAGGCCATTCAACAGG	CTTTGCGAGAGACCTCTTCC	20	20	50.0	55.0	58.1	58.0	18.9	0.6	0.088056	59318	59851	890-890	0.500000	59.3
psaA_p1	psaA	1	TCGCTCGAGTTCTGAATTGG	CCGGTTGACCAAAATGAGGA	20	20	50.0	50.0	58.0	58.1	15.1	0.0	0.099422	59852	62101	2712-2712	0.500000	59.2
psaA_p2	psaA	2	TCGCTCGAGTTCTGAATTGG	ACTAAATGTCCCGTCCAAGC	20	20	50.0	50.0	58.0	57.9	15.1	0.0	0.113133	59852	62101	2998-2998	0.500000	59.1
psaA_p3	psaA	3	TCGCTCGAGTTCTGAATTGG	ACCACCACTGATAAACACCG	20	20	50.0	50.0	58.0	57.8	15.1	0.0	0.182620	59852	62101	2787-2787	0.500000	58.5
psaA_p4	psaA	4	TCGCTCGAGTTCTGAATTGG	CTCGGGTAAAGGCTTCTACG	20	20	50.0	55.0	58.0	57.8	15.1	0.0	0.209015	59852	62101	2733-2733	0.500000	58.3
psaA_p5	psaA	5	CGGTCCATTGAGCACCTATC	CCGGTTGACCAAAATGAGGA	20	20	55.0	50.0	58.1	58.1	0.0	0.0	0.224633	59852	62101	2786-2786	0.500000	58.2
rps14_p1	rps14	1	TTGAAACTCTTGCATGGGCT	AAGGTTATGAGCCTTGCGAG	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.030190	64437	64739	775-775	0.500000	59.8
rps14_p2	rps14	2	TTGAAACTCTTGCATGGGCT	GAACCCGTGTCTTCAAGGTT	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.037180	64437	64739	789-789	0.500000	59.7
rps14_p3	rps14	3	TTGAAACTCTTGCATGGGCT	CAAATGGGGTACATCGAGCA	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.037309	64437	64739	690-690	0.500000	59.7
rps14_p4	rps14	4	TTGAAACTCTTGCATGGGCT	TTGAACCCGTGTCTTCAAGG	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.038558	64437	64739	791-791	0.500000	59.7
rps14_p5	rps14	5	TTGAAACTCTTGCATGGGCT	ACATCGAGCAATCCTTTCCC	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.044475	64437	64739	680-680	0.500000	59.6
psbC_p1	psbC	1	GGCTCTGGTCTTGAAAACCA	GAGATTCGTGCAGCAGAAGA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.020555	65932	67353	1725-1725	0.500000	59.8
psbC_p2	psbC	2	TGGCTCTGGTCTTGAAAACC	GAGATTCGTGCAGCAGAAGA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.020555	65932	67353	1726-1726	0.500000	59.8
psbC_p3	psbC	3	GGCTCTGGTCTTGAAAACCA	GTGCTGCTCTTCTATGTGCT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.040099	65932	67353	2012-2012	0.500000	59.7
psbC_p4	psbC	4	TGGCTCTGGTCTTGAAAACC	GTGCTGCTCTTCTATGTGCT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.040099	65932	67353	2013-2013	0.500000	59.7
psbC_p5	psbC	5	GGCTCTGGTCTTGAAAACCA	GTTGGAGTAGTTGGCCTAGC	20	20	50.0	55.0	58.0	58.0	0.0	3.3	0.044332	65932	67353	1779-1779	0.500000	59.6
psbD_p1	psbD	1	AGTGTCCACAATTTCCCCAC	AGCAGGGAGAAAGAAAAGCT	20	20	50.0	45.0	58.0	57.0	0.0	0.0	0.972368	67301	68362	1613-1613	0.500000	52.2
psbD_p2	psbD	2	CCCACGCAAAAGGTTTTGTT	AGCAGGGAGAAAGAAAAGCT	20	20	45.0	45.0	58.0	57.0	14.9	0.0	0.984883	67301	68362	2067-2067	0.500000	52.1
psbD_p3	psbD	3	GGGTCCAATTAGTGCGTGAT	AGCAGGGAGAAAGAAAAGCT	20	20	50.0	45.0	58.0	57.0	0.0	0.0	1.006132	67301	68362	1700-1700	0.500000	52.0
psbD_p4	psbD	4	AGTGGTTTCTTGGTCACGAC	AGCAGGGAGAAAGAAAAGCT	20	20	50.0	45.0	58.1	57.0	0.0	0.0	1.018279	67301	68362	1382-1382	0.500000	51.9
psbD_p5	psbD	5	CAGTGGTTTCTTGGTCACGA	AGCAGGGAGAAAGAAAAGCT	20	20	50.0	45.0	58.1	57.0	0.0	0.0	1.019640	67301	68362	1383-1383	0.500000	51.8
psbD-trnD-GUC_p1	psbD-trnD-GUC	1	AGCACATAGAAGAGCAGCAC	TTAGAGTACCGCCCTGTCAA	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.093868	68363	69558	1890-1890	0.500000	59.2
psbD-trnD-GUC_p2	psbD-trnD-GUC	2	AGCACATAGAAGAGCAGCAC	CCGGCACAGTTGAATGACTA	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.114661	68363	69558	1951-1951	0.500000	59.1
psbD-trnD-GUC_p3	psbD-trnD-GUC	3	CCAATACTCCAGCAACTCCC	TTAGAGTACCGCCCTGTCAA	20	20	55.0	50.0	58.0	58.1	0.0	0.0	0.116635	68363	69558	1870-1870	0.500000	59.1
psbD-trnD-GUC_p4	psbD-trnD-GUC	4	CCCAATACTCCAGCAACTCC	TTAGAGTACCGCCCTGTCAA	20	20	55.0	50.0	58.0	58.1	0.0	0.0	0.116635	68363	69558	1871-1871	0.500000	59.1
psbD-trnD-GUC_p5	psbD-trnD-GUC	5	TACTCCAGCAACTCCCATCA	TTAGAGTACCGCCCTGTCAA	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.121303	68363	69558	1866-1866	0.500000	59.0
trnE-UUC-psaM_p1	trnE-UUC-psaM	1	TTGACAGGGCGGTACTCTAA	ACAAACAAAGCCTCAAAGCG	20	20	50.0	45.0	58.1	57.8	0.0	0.0	0.280838	69974	70414	1113-1113	0.500000	57.8
trnE-UUC-psaM_p2	trnE-UUC-psaM	2	TAGTCATTCAACTGTGCCGG	ACAAACAAAGCCTCAAAGCG	20	20	50.0	45.0	57.9	57.8	0.0	0.0	0.301632	69974	70414	1052-1052	0.500000	57.6
trnE-UUC-psaM_p3	trnE-UUC-psaM	3	TGGATTTGAACCAGCGTAGG	ACAAACAAAGCCTCAAAGCG	20	20	50.0	45.0	57.9	57.8	0.0	0.0	0.316276	69974	70414	951-951	0.500000	57.5
trnE-UUC-psaM_p4	trnE-UUC-psaM	4	TTGACAGGGCGGTACTCTAA	TTTCTCTAGCCTGGCCAGTA	20	20	50.0	50.0	58.1	57.8	0.0	30.6	0.320212	69974	70414	982-982	0.500000	57.4
trnE-UUC-psaM_p5	trnE-UUC-psaM	5	AGGGCGGTACTCTAACCAAT	ACAAACAAAGCCTCAAAGCG	20	20	50.0	45.0	58.1	57.8	0.0	0.0	0.332838	69974	70414	1108-1108	0.500000	57.3
ndhJ_p1	ndhJ	1	CCAAATACTGGCCAGGCTAG	GTTCGCGGAGTGGATAAGTT	20	20	55.0	50.0	58.0	58.0	35.4	6.1	0.033098	71226	71750	1584-1584	0.500000	59.7
ndhJ_p2	ndhJ	2	CCAAATACTGGCCAGGCTAG	TTTGGTGCCAAGATCCAGTC	20	20	55.0	50.0	58.0	58.1	35.4	3.7	0.117428	71226	71750	1765-1765	0.500000	59.1
ndhJ_p3	ndhJ	3	CCAAATACTGGCCAGGCTAG	CTACTTGCCAGGTTGTCCTC	20	20	55.0	55.0	58.0	57.9	35.4	0.0	0.127733	71226	71750	1549-1549	0.500000	59.0
ndhJ_p4	ndhJ	4	CCAAATACTGGCCAGGCTAG	CCGGAACTGTGACCATGAAA	20	20	55.0	50.0	58.0	58.1	35.4	0.0	0.136252	71226	71750	1718-1718	0.500000	58.9
ndhJ_p5	ndhJ	5	CCAAATACTGGCCAGGCTAG	TAGGCAAGCCGACCTACTTA	20	20	55.0	50.0	58.0	57.9	35.4	5.3	0.175573	71226	71750	1744-1744	0.500000	58.6
ndhC-chlN_p1	ndhC-chlN	1	TTAAGACCATTCCAGCGCTC	GCTCCTTTTCCTATCGGTCC	20	20	50.0	55.0	58.0	57.8	7.1	0.0	0.266222	72923	74068	2082-2082	0.500000	57.9
ndhC-chlN_p2	ndhC-chlN	2	TTAAGACCATTCCAGCGCTC	TGAAGGAAAGGGAGGAACGA	20	20	50.0	50.0	58.0	58.3	7.1	0.0	0.299631	72923	74068	2000-2000	0.500000	57.6
ndhC-chlN_p3	ndhC-chlN	3	TTAAGACCATTCCAGCGCTC	TAGCTATCACGGGAATGGCT	20	20	50.0	50.0	58.0	58.3	7.1	14.8	0.307144	72923	74068	1700-1700	0.500000	57.5
ndhC-chlN_p4	ndhC-chlN	4	GACTGGATCTTGGCACCAAA	GCTCCTTTTCCTATCGGTCC	20	20	50.0	55.0	58.1	57.8	3.3	0.0	0.332347	72923	74068	2346-2346	0.500000	57.3
ndhC-chlN_p5	ndhC-chlN	5	GTCACAGTTCCGGCTGTTAT	GCTCCTTTTCCTATCGGTCC	20	20	50.0	55.0	57.9	57.8	11.6	0.0	0.336809	72923	74068	2386-2386	0.500000	57.3
chlN_p1	chlN	1	TCCCCAACTACCAGCACTAA	GCTCCGTTGAACTATGCAGA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.043110	74069	75427	2319-2319	0.500000	59.7
chlN_p2	chlN	2	TACATCCCCAACTACCAGCA	GCTCCGTTGAACTATGCAGA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.050435	74069	75427	2323-2323	0.500000	59.6
chlN_p3	chlN	3	TCCCCAACTACCAGCACTAA	GTGGTTTGTGGAGGTTTTGC	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.098376	74069	75427	2340-2340	0.500000	59.2
chlN_p4	chlN	4	TACATCCCCAACTACCAGCA	GTGGTTTGTGGAGGTTTTGC	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.105702	74069	75427	2344-2344	0.500000	59.2
chlN_p5	chlN	5	TCCCCAACTACCAGCACTAA	TGTAGAAGCCTGTCCAATGC	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.146825	74069	75427	2146-2146	0.500000	58.8
chlL_p1	chlL	1	TGGACGGCAAAGATCCAAAA	TTGCTTGGCTTCCTCCATTT	20	20	45.0	45.0	58.0	58.0	0.0	0.0	0.025716	75449	76321	1664-1664	0.500000	59.8
chlL_p2	chlL	2	TGGACGGCAAAGATCCAAAA	TTTGCTTGGCTTCCTCCATT	20	20	45.0	45.0	58.0	58.0	0.0	0.0	0.025716	75449	76321	1665-1665	0.500000	59.8
chlL_p3	chlL	3	TTGGACGGCAAAGATCCAAA	TTGCTTGGCTTCCTCCATTT	20	20	45.0	45.0	58.0	58.0	2.5	0.0	0.025716	75449	76321	1665-1665	0.500000	59.8
chlL_p4	chlL	4	TTGGACGGCAAAGATCCAAA	TTTGCTTGGCTTCCTCCATT	20	20	45.0	45.0	58.0	58.0	2.5	0.0	0.025716	75449	76321	1666-1666	0.500000	59.8
chlL_p5	chlL	5	TTCTAGTTTGGGCGCCATAC	TTGCTTGGCTTCCTCCATTT	20	20	50.0	45.0	58.0	58.0	30.8	0.0	0.046690	75449	76321	1435-1435	0.500000	59.6
accD_p1	accD	1	AAAGGACGTGTTATCTCGGC	AGTGTGGACTATTCGCGAAC	20	20	50.0	50.0	58.0	58.0	0.0	26.0	0.013801	102875	104908	2670-2670	0.500000	59.9
accD_p2	accD	2	AAAGGACGTGTTATCTCGGC	TATTCGCGAACGTGGTTCTT	20	20	50.0	45.0	58.0	57.9	0.0	26.0	0.074807	102875	104908	2661-2661	0.500000	59.4
accD_p3	accD	3	CAAGAGCGTAGTCCTGCTTT	AGTGTGGACTATTCGCGAAC	20	20	50.0	50.0	57.9	58.0	0.0	26.0	0.093885	102875	104908	2453-2453	0.500000	59.2
accD_p4	accD	4	AAAGGACGTGTTATCTCGGC	GCTAACGGAGAAAGAGGCAA	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.100733	102875	104908	2891-2891	0.500000	59.2
accD_p5	accD	5	GTGTTTTGGTTTGGTTGGCA	AGTGTGGACTATTCGCGAAC	20	20	45.0	50.0	57.9	58.0	0.0	26.0	0.108435	102875	104908	2842-2842	0.500000	59.1
trnfM-CAU-rbcL_p1	trnfM-CAU-rbcL	1	AACCTTGAAGACACGGGTTC	CCAATCGGGTCGCTTTAGAA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.055370	106304	106880	787-871	1.000000	91.0
trnfM-CAU-rbcL_p2	trnfM-CAU-rbcL	2	AACCTTGAAGACACGGGTTC	ACGGGTGAGATTAAGGGACA	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.089843	106304	106880	1349-1433	1.000000	86.7
trnfM-CAU-rbcL_p3	trnfM-CAU-rbcL	3	CCTTGAAGACACGGGTTCAA	CCAATCGGGTCGCTTTAGAA	20	20	50.0	50.0	58.0	58.0	4.9	0.0	0.056748	106304	106880	785-785	0.500000	59.5
trnfM-CAU-rbcL_p4	trnfM-CAU-rbcL	4	AACCCCATTGCCGATTTCTT	CCAATCGGGTCGCTTTAGAA	20	20	45.0	50.0	58.1	58.0	0.0	0.0	0.081351	106304	106880	1383-1383	0.500000	59.3
trnfM-CAU-rbcL_p5	trnfM-CAU-rbcL	5	ACCCCATTGCCGATTTCTTT	CCAATCGGGTCGCTTTAGAA	20	20	45.0	50.0	58.1	58.0	0.0	0.0	0.081351	106304	106880	1382-1382	0.500000	59.3
psbJ-trnK-UUU_p1	psbJ-trnK-UUU	1	CCGGAAGAATCCCTCTTTGG	AGAGCGGATAATCCACTCCA	20	20	55.0	50.0	58.0	57.9	0.0	0.0	0.132227	109801	110253	610-619	1.000000	86.1
psbJ-trnK-UUU_p2	psbJ-trnK-UUU	2	CCGGAAGAATCCCTCTTTGG	ATCCACTCCATCCGATGAGT	20	20	55.0	50.0	58.0	57.9	0.0	10.1	0.138223	109801	110253	600-609	1.000000	86.1
psbJ-trnK-UUU_p3	psbJ-trnK-UUU	3	CCGGAAGAATCCCTCTTTGG	GCGGATAATCCACTCCATCC	20	20	55.0	55.0	58.0	57.9	0.0	0.0	0.144727	109801	110253	607-616	1.000000	86.0
psbJ-trnK-UUU_p4	psbJ-trnK-UUU	4	CCGGAAGAATCCCTCTTTGG	TCCACTCCATCCGATGAGTT	20	20	55.0	50.0	58.0	58.1	0.0	10.1	0.178108	109801	110253	599-608	1.000000	85.8
psbJ-trnK-UUU_p5	psbJ-trnK-UUU	5	CCGGAAGAATCCCTCTTTGG	CACTCCATCCGATGAGTTCC	20	20	55.0	55.0	58.0	57.8	0.0	10.1	0.275337	109801	110253	597-606	1.000000	85.0
trnK-UUU-chlB_p1	trnK-UUU-chlB	1	AGTCGCACTTAAAAGCCGAG	TCGAAGTAATGTACGCAGCT	20	20	50.0	45.0	58.6	57.1	6.4	0.0	1.504032	112660	113599	1026-1178	1.000000	75.4
trnK-UUU-chlB_p2	trnK-UUU-chlB	2	AGTCGCACTTAAAAGCCGAG	GTCGAAGTAATGTACGCAGC	20	20	50.0	50.0	58.6	57.0	6.4	0.0	1.543692	112660	113599	1027-1179	1.000000	75.1
trnK-UUU-chlB_p3	trnK-UUU-chlB	3	TAGTCGCACTTAAAAGCCGA	TCGAAGTAATGTACGCAGCT	20	20	45.0	45.0	57.3	57.1	3.0	0.0	1.662185	112660	113599	1027-1179	1.000000	74.2
trnK-UUU-chlB_p4	trnK-UUU-chlB	4	TCGCACTTAAAAGCCGAGTA	TCGAAGTAATGTACGCAGCT	20	20	45.0	45.0	57.3	57.1	3.0	0.0	1.662185	112660	113599	1024-1176	1.000000	74.2
trnK-UUU-chlB_p5	trnK-UUU-chlB	5	TCGCACTTAAAAGCCGAGTA	GTCGAAGTAATGTACGCAGC	20	20	45.0	50.0	57.3	57.0	3.0	0.0	1.701845	112660	113599	1025-1177	1.000000	73.9