Hotspot

ndhF

SSC · 106989-109178 · 2190 bp

Back to Callitriche

Pi 0.0394

MarkerSeek score 60.40

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2190
variable_sites: 158
pi: 0.0394
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	GGCTATCAAAACGGAACCCT	GGGGGTTGGTTGGATCTTTC	50.0	55.0	57.9	58.4	2923-2948 bp	0.75	69.3
ndhF_p2	AGGCTATCAAAACGGAACCC	AAAAGGGGGTTGGTTGGATC	50.0	50.0	57.9	58.0	2928 bp	0.5	58.8
ndhF_p3	GGCTATCAAAACGGAACCCT	AAAAGGGGGTTGGTTGGATC	50.0	50.0	57.9	58.0	2927 bp	0.5	58.8
ndhF_p4	TCGAGTTACAGAGACAGGCT	AAAAGGGGGTTGGTTGGATC	50.0	50.0	57.8	58.0	2943 bp	0.5	58.2
ndhF_p5	ATGGAATCGACCATTTCGCT	AAAAGGGGGTTGGTTGGATC	45.0	50.0	57.7	58.0	2775 bp	0.5	57.2