Hotspot

matK

LSC · 1906-3435 · 1530 bp

Back to Callitriche

Pi 0.0418

MarkerSeek score 72.90

Primer available yes

Label matK

Feature Metadata

label_name: matK
feature_type: gene
region: LSC
length_bp: 1530
variable_sites: 118
pi: 0.0418
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK_p1	TTCGATATGCCAGTGGGTTG	AAGACCACGACTGATCCTGA	50.0	50.0	58.0	58.1	2463-2498 bp	1.0	85.8
matK_p2	TTCGATATGCCAGTGGGTTG	AGACCACGACTGATCCTGAA	50.0	50.0	58.0	58.1	2462-2497 bp	1.0	85.8
matK_p3	TTCGATATGCCAGTGGGTTG	TGAAGACCACGACTGATCCT	50.0	50.0	58.0	58.1	2465-2500 bp	1.0	85.8
matK_p4	TTCGATATGCCAGTGGGTTG	TGGAAAAACGAAAGGGCAGA	50.0	45.0	58.0	57.9	1981-1996 bp	0.75	73.3
matK_p5	CTACTGGAGGAGCAGCAATG	TGGAAAAACGAAAGGGCAGA	55.0	45.0	58.0	57.9	2375-2390 bp	0.75	73.0