Hotspot

ndhE-ndhG

SSC · 114963-115195 · 233 bp

Back to Callitriche

Pi 0.0607

MarkerSeek score 74.60

Primer available yes

Label ndhE-ndhG

Feature Metadata

label_name: ndhE-ndhG
feature_type: igs
region: SSC
length_bp: 233
variable_sites: 26
pi: 0.0607
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 74.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE-ndhG_p1	ACATCCGTCCCAAGGTATCA	ACGTCCTGGTATGGGATCAT	50.0	50.0	58.1	57.9	1066-1075 bp	1.0	85.2
ndhE-ndhG_p2	TGGACAGGCAGTTTCACATC	ACGTCCTGGTATGGGATCAT	50.0	50.0	58.1	57.9	1141-1150 bp	0.75	72.0
ndhE-ndhG_p3	TCTGTTGGACAGGCAGTTTC	ACGTCCTGGTATGGGATCAT	50.0	50.0	58.0	57.9	1146-1151 bp	0.5	59.3
ndhE-ndhG_p4	TCTGTTGGACAGGCAGTTTC	TTTTTCTTGCGCAGGTCAAC	50.0	45.0	58.0	57.8	1566-1571 bp	0.5	58.4
ndhE-ndhG_p5	TCTGTTGGACAGGCAGTTTC	TGTTCGTGAATGGTTCGGAA	50.0	45.0	58.0	57.8	1268-1273 bp	0.5	58.1