Hotspot

ndhD-psaC

SSC · 114065-114160 · 96 bp

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Pi 0.0469

MarkerSeek score 72.40

Primer available yes

Label ndhD-psaC

Feature Metadata

label_name: ndhD-psaC
feature_type: igs
region: SSC
length_bp: 96
variable_sites: 9
pi: 0.0469
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-psaC_p1	CCCCTAATCGCCAATGGAAA	CAAATTGCTTCCGCTCCAAG	50.0	50.0	57.9	58.0	492-493 bp	1.0	85.0
ndhD-psaC_p2	CCCCTAATCGCCAATGGAAA	CCCGCAGTATGGCTTTAAGT	50.0	50.0	57.9	58.0	384 bp	0.75	72.7
ndhD-psaC_p3	CCCCTAATCGCCAATGGAAA	ACCCGCAGTATGGCTTTAAG	50.0	50.0	57.9	58.0	385 bp	0.5	59.4
ndhD-psaC_p4	CCCCTAATCGCCAATGGAAA	AAATTGCTTCCGCTCCAAGA	50.0	45.0	57.9	58.1	492 bp	0.5	58.9
ndhD-psaC_p5	CCCCTAATCGCCAATGGAAA	AATTGCTTCCGCTCCAAGAA	50.0	45.0	57.9	58.1	491 bp	0.5	58.9