Hotspot

trnL-UAG-ccsA

SSC · 111306-111408 · 103 bp

Back to Callitriche

Pi 0.0469

MarkerSeek score 72.20

Primer available yes

Label trnL-UAG-ccsA

Feature Metadata

label_name: trnL-UAG-ccsA
feature_type: igs
region: SSC
length_bp: 103
variable_sites: 8
pi: 0.0469
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 72.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAG-ccsA_p1	TGATAGCAGCGAGGGTAGAA	AAAAGGGCTGCATAACCGAA	50.0	45.0	57.9	58.1	1143-1194 bp	0.5	58.9
trnL-UAG-ccsA_p2	TTGATAGCAGCGAGGGTAGA	AAAAGGGCTGCATAACCGAA	50.0	45.0	57.9	58.1	1144-1195 bp	0.5	58.9
trnL-UAG-ccsA_p3	GAAGAGTTCAAGCAGGGGAG	AAAAGGGCTGCATAACCGAA	55.0	45.0	57.9	58.1	829-830 bp	0.5	58.6
trnL-UAG-ccsA_p4	TAGACACGCTGCTCTTAGGA	AAAAGGGCTGCATAACCGAA	50.0	45.0	57.9	58.1	615-616 bp	0.5	58.5
trnL-UAG-ccsA_p5	TGATAGCAGCGAGGGTAGAA	AAAGGGCTGCATAACCGAAT	50.0	45.0	57.9	57.9	1142-1193 bp	0.5	58.4