Hotspot

rpl32-trnL-UAG

SSC · 110109-111225 · 1117 bp

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Pi 0.0763

MarkerSeek score 75.40

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 1117
variable_sites: 107
pi: 0.0763
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.72
markerseek_score: 75.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GATCCAACCAACCCCCTTTT	AAAAGGGCTGCATAACCGAA	50.0	45.0	58.0	58.1	1925-2167 bp	0.5	63.1
rpl32-trnL-UAG_p2	GATCCAACCAACCCCCTTTT	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.0	57.9	1330-1571 bp	0.5	62.8
rpl32-trnL-UAG_p3	GATCCAACCAACCCCCTTTT	AAAGGGCTGCATAACCGAAT	50.0	45.0	58.0	57.9	1924-2166 bp	0.5	62.7
rpl32-trnL-UAG_p4	GATCCAACCAACCCCCTTTT	AGCAGCGTGTCTACCAATTT	50.0	45.0	58.0	57.8	1323-1564 bp	0.5	62.3
rpl32-trnL-UAG_p5	GATCCAACCAACCCCCTTTT	CTAAGAGCAGCGTGTCTACC	50.0	55.0	58.0	57.8	1328-1569 bp	0.5	62.2