Hotspot

ndhF-rpl32

SSC · 109179-109943 · 765 bp

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Pi 0.0686
MarkerSeek score 73.60
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
765
variable_sites
91
pi
0.0686
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
73.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 TGGTTGCTGTCGGAAAAAGA GGAAAACGCCTTTAACGCTG 45.0 50.0 58.0 58.3 682-964 bp 0.75 75.9
ndhF-rpl32_p2 GCAACAGCACCAGCAAATAG CCCGGTCGAAAGAGATTTCC 50.0 55.0 58.0 58.3 1596-1604 bp 0.75 71.9
ndhF-rpl32_p3 GCAACAGCACCAGCAAATAG CCGGTCGAAAGAGATTTCCC 50.0 55.0 58.0 58.3 1595-1603 bp 0.75 71.9
ndhF-rpl32_p4 GCAACAGCACCAGCAAATAG CCCCACCACGGAAAGAATTT 50.0 50.0 58.0 58.4 1862-2155 bp 0.5 60.9
ndhF-rpl32_p5 GCAACAGCACCAGCAAATAG GGAAAACGCCTTTAACGCTG 50.0 50.0 58.0 58.3 1580 bp 0.5 57.3