Hotspot

accD-psaI

LSC · 57009-57709 · 701 bp

Back to Callitriche

Pi 0.0499

MarkerSeek score 73.90

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 701
variable_sites: 64
pi: 0.0499
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 73.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	GCATGCAAGAAGGAAGCTTG	TCGCGGAAATCACACGTTAT	50.0	45.0	58.0	58.0	1224-1284 bp	1.0	88.3
accD-psaI_p2	GCATGCAAGAAGGAAGCTTG	TTTTTCGCGGAAATCACACG	50.0	45.0	58.0	58.0	1228-1288 bp	1.0	88.2
accD-psaI_p3	GCATGCAAGAAGGAAGCTTG	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.0	58.0	1128-1146 bp	1.0	88.1
accD-psaI_p4	GCATGCAAGAAGGAAGCTTG	CGCGGAAATCACACGTTATG	50.0	50.0	58.0	57.9	1223-1283 bp	1.0	87.7
accD-psaI_p5	GACGGGATTAACTGAGGCTG	TCGCGGAAATCACACGTTAT	55.0	45.0	58.1	58.0	1418-1478 bp	1.0	87.1