Hotspot

rps2-rpoC2

LSC · 15610-15844 · 235 bp

Back to Callitriche

Pi 0.0511

MarkerSeek score 77.80

Primer available yes

Label rps2-rpoC2

Feature Metadata

label_name: rps2-rpoC2
feature_type: igs
region: LSC
length_bp: 235
variable_sites: 21
pi: 0.0511
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2-rpoC2_p1	CATTTCCTAGTCCCATGGCC	GACCCCTTCAAAACAGCAGA	55.0	50.0	58.0	58.0	448-475 bp	1.0	89.2
rps2-rpoC2_p2	CCGCATCTCTTTTCGGAAGA	GACCCCTTCAAAACAGCAGA	50.0	50.0	58.0	58.0	783-810 bp	1.0	86.5
rps2-rpoC2_p3	CATTTCCTAGTCCCATGGCC	TATAACCCAGGGGCTTCCAA	55.0	50.0	58.0	58.0	1021-1048 bp	1.0	85.2
rps2-rpoC2_p4	CCATTTCCTAGTCCCATGGC	TATAACCCAGGGGCTTCCAA	55.0	50.0	58.0	58.0	1022-1049 bp	1.0	85.2
rps2-rpoC2_p5	CCGCATCTCTTTTCGGAAGA	TATAACCCAGGGGCTTCCAA	50.0	50.0	58.0	58.0	1356-1383 bp	1.0	84.3