Hotspot

rpl32-trnL-UAG

SSC · 120882-121739 · 858 bp

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Pi 0.0795

MarkerSeek score 85.40

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 858
variable_sites: 99
pi: 0.0795
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 85.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	AGCGAAATCACTGTCCACTG	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.2	57.9	976-1051 bp	0.666667	71.0
rpl32-trnL-UAG_p2	AGCGAAATCACTGTCCACTG	AGCAGCGTGTCTACCAATTT	50.0	45.0	58.2	57.8	969-1044 bp	0.666667	70.6
rpl32-trnL-UAG_p3	AGCGAAATCACTGTCCACTG	CTAAGAGCAGCGTGTCTACC	50.0	55.0	58.2	57.8	974-1049 bp	0.666667	70.5
rpl32-trnL-UAG_p4	AGCGAAATCACTGTCCACTG	AGACCCGCATAAAAGAGCTG	50.0	50.0	58.2	58.0	1611-1676 bp	0.666667	70.3
rpl32-trnL-UAG_p5	AGCGAAATCACTGTCCACTG	AGTTAAAAGACCCGACGTGG	50.0	50.0	58.2	57.8	1506 bp	0.333333	50.5