Hotspot

rps3-rpl22

LSC · 89828-90397 · 570 bp

Back to Callisia

Pi 0.176

MarkerSeek score 81.10

Primer available yes

Label rps3-rpl22

Feature Metadata

label_name: rps3-rpl22
feature_type: igs
region: LSC
length_bp: 570
variable_sites: 132
pi: 0.176
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 81.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3-rpl22_p1	TCTAGACCCGCGGAGTAATT	GGTCACAAGTTGGGGGAATT	50.0	50.0	57.9	58.0	1105-1486 bp	1.0	91.4
rps3-rpl22_p2	TCTAGACCCGCGGAGTAATT	TACTTTCGCGAGACATGCAA	50.0	45.0	57.9	57.9	1073-1454 bp	1.0	90.9
rps3-rpl22_p3	TCTAGACCCGCGGAGTAATT	TTACTTTCGCGAGACATGCA	50.0	45.0	57.9	57.9	1074-1455 bp	1.0	90.9
rps3-rpl22_p4	CGATACGCCCTGCTATTTGT	GGTCACAAGTTGGGGGAATT	50.0	50.0	58.1	58.0	1509-1890 bp	1.0	90.6
rps3-rpl22_p5	TCGATACGCCCTGCTATTTG	GGTCACAAGTTGGGGGAATT	50.0	50.0	57.9	58.0	1510-1891 bp	1.0	90.5