Hotspot

petA-psbJ

LSC · 67664-68295 · 632 bp

Back to Callisia

Pi 0.081
MarkerSeek score 85.50
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
632
variable_sites
66
pi
0.081
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
85.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 TGAAAAGGTTCAATTGTACGA TCTGGGTTGGGTTCATCTCT 33.3 50.0 53.7 58.0 615-1093 bp 1.0 50.6
petA-psbJ_p2 TGAAAAGGTTCAATTGTACGA TGGGTTGGGTTCATCTCTCT 33.3 50.0 53.7 58.0 613-1091 bp 1.0 50.6
petA-psbJ_p3 TGAAAAGGTTCAATTGTACGAA TCTGGGTTGGGTTCATCTCT 31.8 50.0 54.5 58.0 615-1093 bp 1.0 50.6
petA-psbJ_p4 TGAAAAGGTTCAATTGTACGAA TGGGTTGGGTTCATCTCTCT 31.8 50.0 54.5 58.0 613-1091 bp 1.0 50.6
petA-psbJ_p5 TTGAAAAGGTTCAATTGTACGA TGGGTTGGGTTCATCTCTCT 31.8 50.0 54.5 58.0 614-1092 bp 1.0 50.6