Hotspot

trnM-CAU-atpE

LSC · 56683-57019 · 337 bp

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Pi 0.124
MarkerSeek score 84.80
Primer available yes
Label trnM-CAU-atpE

Feature Metadata

label_name
trnM-CAU-atpE
feature_type
igs
region
LSC
length_bp
337
variable_sites
51
pi
0.124
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.89
markerseek_score
84.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnM-CAU-atpE_p1 TATTGCTTTCATACGGCGGG CTCTCAGACGAGCTAGGACA 50.0 55.0 58.4 58.0 390-439 bp 1.0 88.5
trnM-CAU-atpE_p2 TATTGCTTTCATACGGCGGG CGAGCTAGGACACGAGTAGA 50.0 55.0 58.4 58.1 382-431 bp 1.0 88.2
trnM-CAU-atpE_p3 TATTGCTTTCATACGGCGGG AGGACACGAGTAGAGGCTTT 50.0 50.0 58.4 58.1 376-425 bp 1.0 88.1
trnM-CAU-atpE_p4 TATTGCTTTCATACGGCGGG TCTCAGACGAGCTAGGACAC 50.0 55.0 58.4 58.3 389-438 bp 1.0 86.6
trnM-CAU-atpE_p5 GTATTGCTTTCATACGGCGG CTCTCAGACGAGCTAGGACA 50.0 55.0 57.3 58.0 391-440 bp 1.0 86.4