Hotspot

petN-psbM

LSC · 31043-31688 · 646 bp

Back to Callisia

Pi 0.0872

MarkerSeek score 84.80

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 646
variable_sites: 72
pi: 0.0872
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 84.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	AGTATGGGGAAGGAGTGGAC	TCTACGTAAAAACAGTCAGTCA	55.0	36.4	58.1	55.1	608-1144 bp	1.0	51.1
petN-psbM_p2	GTATGGGGAAGGAGTGGACT	TCTACGTAAAAACAGTCAGTCA	55.0	36.4	58.1	55.1	607-1143 bp	1.0	51.1
petN-psbM_p3	TATGGGGAAGGAGTGGACTC	TCTACGTAAAAACAGTCAGTCA	55.0	36.4	57.8	55.1	606-1142 bp	1.0	51.1
petN-psbM_p4	AGTATGGGGAAGGAGTGGAC	TGCTACTGCACTGTTTATTT	55.0	35.0	58.1	53.1	655-1191 bp	1.0	50.7
petN-psbM_p5	GTATGGGGAAGGAGTGGACT	TGCTACTGCACTGTTTATTT	55.0	35.0	58.1	53.1	654-1190 bp	1.0	50.7