Hotspot

trnS-GCU-trnG-UCC

LSC · 9701-10343 · 643 bp

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Pi 0.0684
MarkerSeek score 84.30
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
643
variable_sites
61
pi
0.0684
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.98
markerseek_score
84.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 GCTTTAGTCCACTCAGCCAT ACGAATCGCACTTTTACCACT 50.0 42.9 57.6 58.2 662-750 bp 1.0 77.7
trnS-GCU-trnG-UCC_p2 GCTTTAGTCCACTCAGCCAT ACGAATCGCACTTTTACCAC 50.0 45.0 57.6 56.8 662-750 bp 1.0 77.7
trnS-GCU-trnG-UCC_p3 AGTCCACTCAGCCATCTCTC ACGAATCGCACTTTTACCACT 55.0 42.9 58.5 58.2 657-745 bp 1.0 76.9
trnS-GCU-trnG-UCC_p4 AGTCCACTCAGCCATCTCTC ACGAATCGCACTTTTACCAC 55.0 45.0 58.5 56.8 657-745 bp 1.0 76.9
trnS-GCU-trnG-UCC_p5 GCTTTAGTCCACTCAGCCAT AACGAATCGCACTTTTACCAC 50.0 42.9 57.6 57.5 663-751 bp 1.0 75.4