Hotspot

rps16-trnQ-UUG

LSC · 6018-8420 · 2403 bp

Back to Callisia

Pi 0.0886

MarkerSeek score 78.60

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 2403
variable_sites: 237
pi: 0.0886
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.86
markerseek_score: 78.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	CGTTGCTTTCTACCACATCG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.2	58.1	1550-2468 bp	1.0	85.1
rps16-trnQ-UUG_p2	CGTTGCTTTCTACCACATCG	TTTAAACAGTGATGGGGCGT	50.0	45.0	57.2	57.7	1610-2528 bp	1.0	83.5
rps16-trnQ-UUG_p3	CGTTGCTTTCTACCACATCG	TTAAACAGTGATGGGGCGTG	50.0	50.0	57.2	58.5	1609-2527 bp	1.0	81.9
rps16-trnQ-UUG_p4	CGTTGCTTTCTACCACATCG	TTCGGAGGTTCGAATCCTTC	50.0	50.0	57.2	57.3	1554-2472 bp	1.0	80.4
rps16-trnQ-UUG_p5	CGTTGCTTTCTACCACATCG	TAAGGCAACGGGTTTTGGTC	50.0	50.0	57.2	58.7	1580-2498 bp	1.0	80.3