Hotspot

rps11

LSC · 81860-82276 · 417 bp

Back to Calligonum

Pi 0.00248

MarkerSeek score 35.00

Primer available yes

Label rps11

Feature Metadata

label_name: rps11
feature_type: gene
region: LSC
length_bp: 417
variable_sites: 6
pi: 0.00248
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 35.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps11_p1	TCCCTTCACTTCAATGCTTAGT	TCTCCATATATCTCTGACTCA	40.9	38.1	57.4	52.0	486 bp	1.0	41.0
rps11_p2	TGAAATCCCTTCACTTCAATGC	TCTCCATATATCTCTGACTCA	40.9	38.1	57.2	52.0	491 bp	1.0	41.0
rps11_p3	TCCCTTCACTTCAATGCTTAGT	TGGATATCTCCATATATCTCTGACTCA	40.9	37.0	57.4	58.2	492 bp	1.0	41.0
rps11_p4	TCCCTTCACTTCAATGCTTAGT	GGATATCTCCATATATCTCTGACTCA	40.9	38.5	57.4	56.8	491 bp	1.0	41.0
rps11_p5	TCCCTTCACTTCAATGCTTAGT	TGGATATCTCCATATATCTCTGACTC	40.9	38.5	57.4	56.8	492 bp	1.0	41.0