Hotspot

rpl16

LSC · 84034-85416 · 1383 bp

Back to Calligonum

Pi 0.00233

MarkerSeek score 52.30

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1383
variable_sites: 23
pi: 0.00233
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	CCAAAACAAAAACTGGCGGT	AAATTCGATTGACCCCCGAC	45.0	50.0	58.0	58.3	1459-1473 bp	1.0	79.8
rpl16_p2	CCAAAACAAAAACTGGCGGT	AAAATTCGATTGACCCCCGA	45.0	45.0	58.0	57.5	1460-1474 bp	1.0	77.8
rpl16_p3	TCCAAAACAAAAACTGGCGG	AAATTCGATTGACCCCCGAC	45.0	50.0	57.7	58.3	1460-1474 bp	1.0	77.5
rpl16_p4	CCAAAACAAAAACTGGCGGT	ATTGACCCCCGACCTAATTG	45.0	50.0	58.0	57.3	1452-1466 bp	1.0	76.0
rpl16_p5	TCCAAAACAAAAACTGGCGG	AAAATTCGATTGACCCCCGA	45.0	45.0	57.7	57.5	1461-1475 bp	1.0	75.5