Hotspot

ndhF-rpl32

SSC · 119558-120540 · 983 bp

Back to Calligonum

Pi 0.00373

MarkerSeek score 57.30

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 983
variable_sites: 25
pi: 0.00373
unique_haplotype_count: 17
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	AAAGAAGAAGTCCCGTTCCA	TGCTGCCGAATATCCTTTTCT	45.0	42.9	56.7	57.7	1152-1155 bp	1.0	61.4
ndhF-rpl32_p2	AAGAAGAAGTCCCGTTCCAA	TGCTGCCGAATATCCTTTTCT	45.0	42.9	56.7	57.7	1151-1154 bp	1.0	61.4
ndhF-rpl32_p3	TCGGAAAAAGAAGAAGTCCCG	TGCTGCCGAATATCCTTTTCT	47.6	42.9	58.2	57.7	1158-1161 bp	0.952381	59.9
ndhF-rpl32_p4	CGGAAAAAGAAGAAGTCCCG	TGCTGCCGAATATCCTTTTCT	50.0	42.9	56.7	57.7	1157-1160 bp	0.952381	59.8
ndhF-rpl32_p5	TCGGAAAAAGAAGAAGTCCCG	AAGCTTTTAATGCTGCCGAA	47.6	40.0	58.2	56.6	1168-1171 bp	0.952381	59.0