Hotspot

trnR-ACG-trnN-GUU

IRb · 110466-111076 · 611 bp

Back to Calligonum

Pi 0.00338
MarkerSeek score 51.40
Primer available yes
Label trnR-ACG-trnN-GUU

Feature Metadata

label_name
trnR-ACG-trnN-GUU
feature_type
igs
region
IRb
length_bp
611
variable_sites
9
pi
0.00338
unique_haplotype_count
10
primer_available
yes
alignment_reliability
1.00
markerseek_score
51.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-ACG-trnN-GUU_p1 CAGAGGATTAGAGCACGTGG GCTGTTAACTGACTGGTCGT 55.0 50.0 57.8 57.8 707-721 bp 1.0 79.1
trnR-ACG-trnN-GUU_p2 CAGAGGATTAGAGCACGTGG TGGTCGTAGGTTCGAATCCT 55.0 50.0 57.8 58.2 694-708 bp 1.0 79.1
trnR-ACG-trnN-GUU_p3 CAGAGGATTAGAGCACGTGG CTGGTCGTAGGTTCGAATCC 55.0 55.0 57.8 57.8 695-709 bp 1.0 78.7
trnR-ACG-trnN-GUU_p4 CAGAGGATTAGAGCACGTGG CGTTCCTCAGTAGCTCAGTG 55.0 55.0 57.8 57.7 739-753 bp 1.0 78.0
trnR-ACG-trnN-GUU_p5 CAGAGGATTAGAGCACGTGG TTCCTCAGTAGCTCAGTGGT 55.0 50.0 57.8 57.7 737-751 bp 1.0 77.7