Hotspot

psaJ-rpl33

LSC · 69545-70057 · 513 bp

Back to Calligonum

Pi 0.00387

MarkerSeek score 53.90

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 513
variable_sites: 10
pi: 0.00387
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 53.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	GGATGCGTTAACATTCCCCT	GGACATCTTTACCCTTGGCC	50.0	55.0	57.9	58.2	558-596 bp	1.0	82.4
psaJ-rpl33_p2	GGATGCGTTAACATTCCCCT	CACGCTTACTCGGACATCTT	50.0	50.0	57.9	57.7	569-607 bp	1.0	82.0
psaJ-rpl33_p3	TTTTCCCGGATGCGTTAACA	GGACATCTTTACCCTTGGCC	45.0	55.0	58.1	58.2	565-603 bp	1.0	82.0
psaJ-rpl33_p4	TTCCCGGATGCGTTAACATT	GGACATCTTTACCCTTGGCC	45.0	55.0	57.9	58.2	563-601 bp	1.0	81.9
psaJ-rpl33_p5	TTTCCCGGATGCGTTAACAT	GGACATCTTTACCCTTGGCC	45.0	55.0	57.9	58.2	564-602 bp	1.0	81.9