Hotspot

psbE-petL

LSC · 66880-68045 · 1166 bp

Back to Calligonum

Pi 0.000804

MarkerSeek score 52.50

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1166
variable_sites: 7
pi: 0.000804
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	AGAACGTTCTCCTGTGCTTC	AAGCCGTATCTTGCTCAGAC	50.0	50.0	57.8	57.7	1251-1293 bp	1.0	79.8
psbE-petL_p2	GTTCTCCTGTGCTTCCAGAC	AAGCCGTATCTTGCTCAGAC	55.0	50.0	58.2	57.7	1246-1288 bp	1.0	79.5
psbE-petL_p3	TGCTGACGAATAACCAACCC	AAGCCGTATCTTGCTCAGAC	50.0	50.0	58.2	57.7	1342-1384 bp	1.0	79.4
psbE-petL_p4	CGCAAAAGAACGTTCTCCTG	AAGCCGTATCTTGCTCAGAC	50.0	50.0	57.7	57.7	1257-1299 bp	1.0	78.7
psbE-petL_p5	GACGAATAACCAACCCGCTA	AAGCCGTATCTTGCTCAGAC	50.0	50.0	57.7	57.7	1338-1380 bp	1.0	78.5