Hotspot

trnC-GCA-petN

LSC · 28273-29155 · 883 bp

Back to Calligonum

Pi 0.00836

MarkerSeek score 62.50

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 883
variable_sites: 70
pi: 0.00836
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTCCCCGGTTCAAATCTGG	CCATACGACGAGTGAAAGGG	50.0	55.0	58.1	58.1	953-1000 bp	1.0	88.5
trnC-GCA-petN_p2	TTTCCCCGGTTCAAATCTGG	CCCATACGACGAGTGAAAGG	50.0	55.0	58.1	58.1	954-1001 bp	1.0	88.4
trnC-GCA-petN_p3	TTTCCCCGGTTCAAATCTGG	CCCCATACGACGAGTGAAAG	50.0	55.0	58.1	58.1	955-1002 bp	1.0	88.4
trnC-GCA-petN_p4	TTTCCCCGGTTCAAATCTGG	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.1	58.2	908-955 bp	1.0	88.2
trnC-GCA-petN_p5	TTTCCCCGGTTCAAATCTGG	AGTCCACTTCTCCCCCATAC	50.0	55.0	58.1	58.1	967-1014 bp	1.0	88.0