Hotspot

rpoB-trnC-GCA

LSC · 23938-28200 · 4263 bp

Back to Calligonum

Pi 0.00138
MarkerSeek score 48.10
Primer available yes
Label rpoB-trnC-GCA

Feature Metadata

label_name
rpoB-trnC-GCA
feature_type
igs
region
LSC
length_bp
4263
variable_sites
44
pi
0.00138
unique_haplotype_count
16
primer_available
yes
alignment_reliability
1.00
markerseek_score
48.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoB-trnC-GCA_p1 GCGGGGGTCTATCCAAATTT TCCCTAATGACATAAGAAAGTCAA 50.0 33.3 57.9 55.5 304-332 bp 1.0 44.9
rpoB-trnC-GCA_p2 GCGGGGGTCTATCCAAATTT TCCCTAATGACATAAGAAAGTCAAA 50.0 32.0 57.9 56.2 304-332 bp 1.0 44.9
rpoB-trnC-GCA_p3 GCGGGGGTCTATCCAAATTT TGTTTCCCTAATGACATAAGAAAGT 50.0 32.0 57.9 56.4 308-336 bp 1.0 44.8
rpoB-trnC-GCA_p4 GCGGGGGTCTATCCAAATTT TGTTTCCCTAATGACATAAGAAAGTC 50.0 34.6 57.9 57.4 308-336 bp 1.0 44.8
rpoB-trnC-GCA_p5 GCGGGGGTCTATCCAAATTT TTCCCTAATGACATAAGAAAGTCAA 50.0 32.0 57.9 56.2 305-333 bp 1.0 44.8