Hotspot

trnK-UUU-rps16

LSC · 4334-5308 · 975 bp

Back to Calligonum

Pi 0.00171

MarkerSeek score 52.60

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 975
variable_sites: 14
pi: 0.00171
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	CGAATCCCTTGCTTCATCCA	ATTTTCTTGAAAGGGGCGCT	50.0	45.0	58.0	58.4	1130-1175 bp	1.0	80.2
trnK-UUU-rps16_p2	ACGAATCCCTTGCTTCATCC	ATTTTCTTGAAAGGGGCGCT	50.0	45.0	58.0	58.4	1131-1176 bp	1.0	80.2
trnK-UUU-rps16_p3	GGACGAATCCCTTGCTTCAT	ATTTTCTTGAAAGGGGCGCT	50.0	45.0	58.0	58.4	1133-1178 bp	1.0	80.2
trnK-UUU-rps16_p4	AACTCTACCGATGGTGTGGA	ATTTTCTTGAAAGGGGCGCT	50.0	45.0	58.1	58.4	1150-1195 bp	1.0	80.0
trnK-UUU-rps16_p5	GACCCTAGCCGCACTTAAAA	ATTTTCTTGAAAGGGGCGCT	50.0	45.0	57.9	58.4	1099-1144 bp	1.0	79.8