Hotspot

ycf1

SSC · 124243-129915 · 5673 bp

Back to Callicarpa

Pi 0.00219

MarkerSeek score 57.20

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5673
variable_sites: 70
pi: 0.00219
unique_haplotype_count: 19
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CGTTTGCCTTTTCTTGAACGA	AAATCCGAAGGGTCCTTCTT	42.9	45.0	57.9	56.4	261 bp	1.0	61.4
ycf1_p2	CGTTTGCCTTTTCTTGAACGA	AATCCGAAGGGTCCTTCTTT	42.9	45.0	57.9	56.4	260 bp	1.0	61.4
ycf1_p3	ACGTTTGCCTTTTCTTGAACG	AAATCCGAAGGGTCCTTCTT	42.9	45.0	58.2	56.4	262 bp	1.0	60.2
ycf1_p4	ACGTTTGCCTTTTCTTGAACG	AATCCGAAGGGTCCTTCTTT	42.9	45.0	58.2	56.4	261 bp	1.0	60.2
ycf1_p5	CGTTTGCCTTTTCTTGAACGA	AAATCCGAAGGGTCCTTCTTT	42.9	42.9	57.9	57.2	261 bp	1.0	59.5