Hotspot

rpl16

LSC · 82345-83639 · 1295 bp

Back to Callicarpa

Pi 0.00228

MarkerSeek score 42.80

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1295
variable_sites: 19
pi: 0.00228
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 42.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	TGCGTTTCATTCCCAAGACT	TCGTTCATTCTTTTCCTGGTCA	45.0	40.9	57.7	57.9	1424 bp	0.05	23.2
rpl16_p2	AAACCTGCGTTTCATTCCCA	TCGTTCATTCTTTTCCTGGTCA	45.0	40.9	58.3	57.9	1429 bp	0.05	22.9
rpl16_p3	AACCTGCGTTTCATTCCCAA	TCGTTCATTCTTTTCCTGGTCA	45.0	40.9	58.3	57.9	1428 bp	0.05	22.9
rpl16_p4	CCTGCGTTTCATTCCCAAGA	TCGTTCATTCTTTTCCTGGTCA	50.0	40.9	58.5	57.9	1426 bp	0.05	21.6
rpl16_p5	CTGCGTTTCATTCCCAAGAC	TCGTTCATTCTTTTCCTGGTCA	50.0	40.9	57.4	57.9	1425 bp	0.05	20.4