Hotspot

rbcL

LSC · 56410-57849 · 1440 bp

Back to Callicarpa

Pi 0.00407

MarkerSeek score 49.00

Primer available yes

Label rbcL

Feature Metadata

label_name: rbcL
feature_type: gene
region: LSC
length_bp: 1440
variable_sites: 29
pi: 0.00407
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL_p1	CGCCTAATTCATTCGTGTCG	TGGGCCGAGTTTAATTGCAA	50.0	45.0	57.4	58.4	1558-1573 bp	0.95	72.5
rbcL_p2	CGCCTAATTCATTCGTGTCG	TTGGGCCGAGTTTAATTGCA	50.0	45.0	57.4	58.4	1559-1574 bp	0.95	72.5
rbcL_p3	TCATGAGTTGTAGGGAGGGA	TGGGCCGAGTTTAATTGCAA	50.0	45.0	57.1	58.4	1507-1522 bp	0.95	70.0
rbcL_p4	TCATGAGTTGTAGGGAGGGA	TTGGGCCGAGTTTAATTGCA	50.0	45.0	57.1	58.4	1508-1523 bp	0.95	70.0
rbcL_p5	TTCATGAGTTGTAGGGAGGGA	TGGGCCGAGTTTAATTGCAA	47.6	45.0	57.8	58.4	1508-1523 bp	0.95	68.0