Hotspot

psbE-petL

LSC · 66156-67077 · 922 bp

Back to Callicarpa

Pi 0.00443

MarkerSeek score 42.40

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 922
variable_sites: 14
pi: 0.00443
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 42.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	AGCCACTAGTAGAAAACCGA	50.0	45.0	57.9	55.9	1068-1090 bp	1.0	64.7
psbE-petL_p2	ACTGACGAATAACCAACCCG	AGCCACTAGTAGAAAACCGA	50.0	45.0	57.9	55.9	1081-1103 bp	1.0	64.4
psbE-petL_p3	AGAACGTTCTCCTGTGCTTC	AGCCACTAGTAGAAAACCGA	50.0	45.0	57.8	55.9	991-1013 bp	1.0	64.0
psbE-petL_p4	ACGAATAACCAACCCGCAAT	AGCCACTAGTAGAAAACCGA	45.0	45.0	58.2	55.9	1077-1099 bp	1.0	63.7
psbE-petL_p5	CGAATAACCAACCCGCAATG	AGCCACTAGTAGAAAACCGA	50.0	45.0	57.8	55.9	1076-1098 bp	1.0	63.6