Hotspot

petA-psbJ

LSC · 64337-65373 · 1037 bp

Back to Callicarpa

Pi 0.00246

MarkerSeek score 54.90

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1037
variable_sites: 19
pi: 0.00246
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 54.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GAGAAGGTTCAATTGGCCGA	TCCGGATTAGGTTCATCCCT	50.0	50.0	58.2	57.5	983-1094 bp	1.0	80.2
petA-psbJ_p2	TCGAGAAGGTTCAATTGGCC	TCCGGATTAGGTTCATCCCT	50.0	50.0	58.2	57.5	985-1096 bp	1.0	80.2
petA-psbJ_p3	GAGAAGGTTCAATTGGCCGA	CCGGATTAGGTTCATCCCTG	50.0	55.0	58.2	57.4	982-1093 bp	1.0	79.7
petA-psbJ_p4	TCGAGAAGGTTCAATTGGCC	CCGGATTAGGTTCATCCCTG	50.0	55.0	58.2	57.4	984-1095 bp	1.0	79.7
petA-psbJ_p5	AGAAGGTTCAATTGGCCGAA	TCCGGATTAGGTTCATCCCT	45.0	50.0	57.7	57.5	982-1093 bp	1.0	79.3