Hotspot

ndhC-trnV-UAC

LSC · 51431-52615 · 1185 bp

Back to Callicarpa

Pi 0.00217
MarkerSeek score 51.40
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1185
variable_sites
16
pi
0.00217
unique_haplotype_count
11
primer_available
yes
alignment_reliability
0.99
markerseek_score
51.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 GGGGCTAAAATCCCGGAAAT AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.9 57.8 1314-1324 bp 1.0 79.7
ndhC-trnV-UAC_p2 CCTGATCGGGGCTAAAATCC AGAAGGTCTACGGTTCGAGT 55.0 50.0 58.1 57.8 1321-1331 bp 1.0 79.5
ndhC-trnV-UAC_p3 CTGATCGGGGCTAAAATCCC AGAAGGTCTACGGTTCGAGT 55.0 50.0 58.1 57.8 1320-1330 bp 1.0 79.5
ndhC-trnV-UAC_p4 GGGGCTAAAATCCCGGAAAT GAAGGTCTACGGTTCGAGTC 50.0 55.0 57.9 57.5 1313-1323 bp 1.0 77.0
ndhC-trnV-UAC_p5 CTGATCGGGGCTAAAATCCC GAAGGTCTACGGTTCGAGTC 55.0 55.0 58.1 57.5 1319-1329 bp 1.0 76.8