Hotspot

trnT-UGU-trnL-UAA

LSC · 47455-48124 · 670 bp

Back to Callicarpa

Pi 0.00255
MarkerSeek score 49.90
Primer available yes
Label trnT-UGU-trnL-UAA

Feature Metadata

label_name
trnT-UGU-trnL-UAA
feature_type
igs
region
LSC
length_bp
670
variable_sites
12
pi
0.00255
unique_haplotype_count
9
primer_available
yes
alignment_reliability
1.00
markerseek_score
49.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-UGU-trnL-UAA_p1 GCGATGCTCTAACCTCTGAG CCGTAGCGTCTACCGATTTC 55.0 55.0 57.9 58.2 730-758 bp 1.0 78.8
trnT-UGU-trnL-UAA_p2 TAGGAAAAAGCCGGCTATCG CCGTAGCGTCTACCGATTTC 50.0 55.0 57.8 58.2 781-809 bp 1.0 77.9
trnT-UGU-trnL-UAA_p3 GGAATCGAACCGATGACCAT CCGTAGCGTCTACCGATTTC 50.0 55.0 57.8 58.2 762-790 bp 1.0 77.9
trnT-UGU-trnL-UAA_p4 GCGATGCTCTAACCTCTGAG CGTCTACCGATTTCGCCATA 55.0 50.0 57.9 57.6 724-752 bp 1.0 77.5
trnT-UGU-trnL-UAA_p5 GCGATGCTCTAACCTCTGAG GGTTTCCATACCAAGGCTCA 55.0 50.0 57.9 57.5 762-790 bp 1.0 76.7