Hotspot

psbM-trnD-GUC

LSC · 30695-31230 · 536 bp

Back to Callicarpa

Pi 0.00317

MarkerSeek score 51.60

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 536
variable_sites: 11
pi: 0.00317
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	AGTAGAAAAGCGGTAGGAACT	TCAATTGGTTAGAGCACCGC	42.9	50.0	56.4	58.6	652-667 bp	1.0	59.7
psbM-trnD-GUC_p2	AGCGGTAGGAACTAGAATGA	TCAATTGGTTAGAGCACCGC	45.0	50.0	55.4	58.6	644-659 bp	1.0	59.6
psbM-trnD-GUC_p3	AAGCGGTAGGAACTAGAATGA	TCAATTGGTTAGAGCACCGC	42.9	50.0	56.2	58.6	645-660 bp	1.0	58.0
psbM-trnD-GUC_p4	AGCGGTAGGAACTAGAATGAA	TCAATTGGTTAGAGCACCGC	42.9	50.0	56.2	58.6	644-659 bp	1.0	58.0
psbM-trnD-GUC_p5	AAGTAGAAAAGCGGTAGGAACT	TCAATTGGTTAGAGCACCGC	40.9	50.0	57.1	58.6	653-668 bp	1.0	57.4