Hotspot

petN-psbM

LSC · 29680-30589 · 910 bp

Back to Callicarpa

Pi 0.00388

MarkerSeek score 58.00

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 910
variable_sites: 26
pi: 0.00388
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	ACTCGTAGTGTGGGGAAGAA	AGTTCCTACCGCTTTTCTACT	50.0	42.9	58.0	56.4	988-1042 bp	1.0	63.7
petN-psbM_p2	TCGTAGTGTGGGGAAGAAGT	AGTTCCTACCGCTTTTCTACT	50.0	42.9	58.0	56.4	986-1040 bp	1.0	63.7
petN-psbM_p3	ACTCGTAGTGTGGGGAAGAA	TCATTCTAGTTCCTACCGCT	50.0	45.0	58.0	55.4	995-1049 bp	1.0	63.5
petN-psbM_p4	TCGTAGTGTGGGGAAGAAGT	TCATTCTAGTTCCTACCGCT	50.0	45.0	58.0	55.4	993-1047 bp	1.0	63.5
petN-psbM_p5	CGTAGTGTGGGGAAGAAGTG	AGTTCCTACCGCTTTTCTACT	55.0	42.9	57.9	56.4	985-1039 bp	1.0	63.1