Hotspot

rpoB-trnC-GCA

LSC · 27512-28687 · 1176 bp

Back to Callicarpa

Pi 0.00227

MarkerSeek score 45.80

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1176
variable_sites: 22
pi: 0.00227
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	GACATTGCCTCGTTTGCATC	TTTTTGGTGATCAGGCGACA	50.0	45.0	58.1	58.0	1287-1288 bp	1.0	81.4
rpoB-trnC-GCA_p2	GACATTGCCTCGTTTGCATC	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.1	58.1	1266-1267 bp	1.0	81.0
rpoB-trnC-GCA_p3	TAGACATTGCCTCGTTTGCA	TTTTTGGTGATCAGGCGACA	45.0	45.0	57.8	58.0	1289-1290 bp	1.0	80.8
rpoB-trnC-GCA_p4	CATTGCCTCGTTTGCATCTC	TTTTTGGTGATCAGGCGACA	50.0	45.0	57.8	58.0	1285-1286 bp	1.0	80.7
rpoB-trnC-GCA_p5	TAGACATTGCCTCGTTTGCA	CCGGATTTGAACTGGGGAAA	45.0	50.0	57.8	58.1	1268-1269 bp	1.0	80.4