Hotspot

ycf1

SSC · 126132-131621 · 5490 bp

Back to Callianthemum

Pi 0.00932

MarkerSeek score 47.10

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5490
variable_sites: 51
pi: 0.00932
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	ATGCCTGTGACAACGAAGAG	CGTGGCACAGATCGAAGTTA	50.0	50.0	58.2	58.0	921-924 bp	1.0	80.2
ycf1_p2	AATTTGTCCAATCCGCGGAT	CGTGGCACAGATCGAAGTTA	45.0	50.0	58.2	58.0	432-435 bp	1.0	80.1
ycf1_p3	ATGCCTGTGACAACGAAGAG	AAATTGGATGGGGAAGGACG	50.0	50.0	58.2	57.9	1316-1319 bp	1.0	78.7
ycf1_p4	AATTTGTCCAATCCGCGGAT	AAATTGGATGGGGAAGGACG	45.0	50.0	58.2	57.9	827-830 bp	1.0	78.2
ycf1_p5	ATGCCTGTGACAACGAAGAG	GGAAGGACGCAGAATTCCAA	50.0	50.0	58.2	58.2	1308 bp	0.5	57.0