Hotspot

psbA

LSC · 279-1340 · 1062 bp

Back to Callianthemum

Pi 0.00282

MarkerSeek score 53.80

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 3
pi: 0.00282
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	TACCCAGCCTCTTGACAGAA	ATTTGTGTGCTTGGGAGTCC	50.0	50.0	58.0	58.4	1200 bp	1.0	77.0
psbA_p2	CTCCTGCAACTTCAACGACT	ATTTGTGTGCTTGGGAGTCC	50.0	50.0	58.1	58.4	1163 bp	1.0	76.1
psbA_p3	TCCTGCAACTTCAACGACTC	ATTTGTGTGCTTGGGAGTCC	50.0	50.0	58.1	58.4	1162 bp	1.0	76.1
psbA_p4	GGGCATTACTCCTGCAACTT	ATTTGTGTGCTTGGGAGTCC	50.0	50.0	58.2	58.4	1171 bp	1.0	75.9
psbA_p5	ATACCCAGCCTCTTGACAGA	ATTTGTGTGCTTGGGAGTCC	50.0	50.0	57.7	58.4	1201 bp	1.0	75.1