Hotspot

trnN-GUU-trnR-ACG

IRa · 132017-132546 · 530 bp

Back to Callianthemum

Pi 0.0132

MarkerSeek score 64.90

Primer available yes

Label trnN-GUU-trnR-ACG

Feature Metadata

label_name: trnN-GUU-trnR-ACG
feature_type: igs
region: IRa
length_bp: 530
variable_sites: 7
pi: 0.0132
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-trnR-ACG_p1	CCGTTGTTCCTCAGTAGCTC	CAGAGGATTAGAGCACGTGG	55.0	55.0	58.0	57.8	671 bp	1.0	79.1
trnN-GUU-trnR-ACG_p2	CCGTTGTTCCTCAGTAGCTC	GAACAGATCCAGTGGAGACG	55.0	55.0	58.0	57.7	708 bp	1.0	78.5
trnN-GUU-trnR-ACG_p3	TGGTCGTAGGTTCGAATCCT	CAGAGGATTAGAGCACGTGG	50.0	55.0	58.2	57.8	622 bp	1.0	77.9
trnN-GUU-trnR-ACG_p4	TGGTCGTAGGTTCGAATCCT	GAACAGATCCAGTGGAGACG	50.0	55.0	58.2	57.7	659 bp	1.0	77.3
trnN-GUU-trnR-ACG_p5	CGGTCGGCTGTTAACTGATT	CAGAGGATTAGAGCACGTGG	50.0	55.0	58.3	57.8	641 bp	1.0	76.9